The Escherichia coli Replisome Is Inherently DNA Damage Tolerant
نویسندگان
چکیده
منابع مشابه
Replisome architecture and dynamics in Escherichia coli.
The field of DNA replication was launched upon discovery of the first DNA polymerase by Kornberg and Lehman (1, 2). DNA polymerase I displayed the novel and highly exciting property of template-directed enzymatic action, and like a split personality, DNApolymerase I could also degradeDNA, from either direction. Escherichia coli is now known to contain five different DNA polymerases. The chromos...
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The structural gene for DNA polymerase II was cloned by using a synthetic inosine-containing oligonucleotide probe corresponding to 11 amino acids, which were determined by sequencing the amino terminus of the purified protein. The labeled oligonucleotide hybridized specifically to the lambda clone 7H9 from the Kohara collection as well as to plasmid pGW511 containing the SOS-regulated dinA gen...
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Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-pr...
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ژورنال
عنوان ژورنال: Science
سال: 2011
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.1209111